Class: ExperimentalCulture

Growth of an experimental culture for downstream analysis.

This is the terminal culture step before plate setup or direct measurement.

__

Input: processedSample(type='pre_culture') via processingSampleLink

Output: processedSample(type='experimental_culture') via processingSampleLink

Refs: Media (growth medium), Strain

 classDiagram
    class ExperimentalCulture
    click ExperimentalCulture href "../ExperimentalCulture/"
      CultureGrowth <|-- ExperimentalCulture
        click CultureGrowth href "../CultureGrowth/"

      ExperimentalCulture : agitation_speed_rpm

      ExperimentalCulture : container_type

      ExperimentalCulture : description

      ExperimentalCulture : growth_medium

      ExperimentalCulture : growth_time

      ExperimentalCulture : id

      ExperimentalCulture : in_protocol





        ExperimentalCulture --> "0..1" SampleProcessingProtocol : in_protocol
        click SampleProcessingProtocol href "../SampleProcessingProtocol/"



      ExperimentalCulture : incubation_time_hours

      ExperimentalCulture : name

      ExperimentalCulture : organism_ref





        ExperimentalCulture --> "0..1" Organism : organism_ref
        click Organism href "../Organism/"



      ExperimentalCulture : oxygen_saturation_pct

      ExperimentalCulture : temperature_celsius

      ExperimentalCulture : treatment_type

Inheritance

SampleProcessing
- CultureGrowth [ HasIncubationConditions]
  - ExperimentalCulture

Slots

Name	Cardinality and Range	Description	Inheritance
treatment_type	0..1 String	Type of treatment applied in experimental culture growth	direct
growth_time	0..1 String	Total growth time for the culture	direct
organism_ref	0..1 Organism	FK reference to an organism representing the biological identity	CultureGrowth
growth_medium	0..1 String	Method of growth and medium/materials used	CultureGrowth
incubation_time_hours	0..1 Float	Incubation duration in hours	CultureGrowth
container_type	0..1 String	Physical container used for the culture or sample (flask, tube, plate, etc	CultureGrowth
temperature_celsius	0..1 Float	Temperature at which the method/process/activity was performed	HasIncubationConditions
agitation_speed_rpm	0..1 Integer	Agitation/shaking speed in RPM (0 for static)	HasIncubationConditions
oxygen_saturation_pct	0..1 Float	Percent saturation of oxygen in the incubation atmosphere (0–100)	HasIncubationConditions
name	1 String	Human-readable name for the entity or activity	SampleProcessing
description	0..1 String	Human-readable description for the entity or activity	SampleProcessing
in_protocol	0..1 SampleProcessingProtocol	The SampleProcessingProtocol (the recipe) that this step follows	SampleProcessing
id	1 Uuid		SampleProcessing

Identifier and Mapping Information

Schema Source

from schema: https://emsl-computing.github.io/BASALT-Schema

Mappings

Mapping Type	Mapped Value
self	basalt_schema:ExperimentalCulture
native	basalt_schema:ExperimentalCulture

LinkML Source

Direct

name: ExperimentalCulture
description: 'Growth of an experimental culture for downstream analysis.

  This is the terminal culture step before plate setup or direct measurement.


  Input:  processedSample(type=''pre_culture'') via processingSampleLink

  Output: processedSample(type=''experimental_culture'') via processingSampleLink

  Refs:   Media (growth medium), Strain'
from_schema: https://emsl-computing.github.io/BASALT-Schema
is_a: CultureGrowth
slots:
- treatment_type
- growth_time

Induced

name: ExperimentalCulture
description: 'Growth of an experimental culture for downstream analysis.

  This is the terminal culture step before plate setup or direct measurement.


  Input:  processedSample(type=''pre_culture'') via processingSampleLink

  Output: processedSample(type=''experimental_culture'') via processingSampleLink

  Refs:   Media (growth medium), Strain'
from_schema: https://emsl-computing.github.io/BASALT-Schema
is_a: CultureGrowth
attributes:
  treatment_type:
    name: treatment_type
    description: Type of treatment applied in experimental culture growth
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: treatment_type
    owner: ExperimentalCulture
    domain_of:
    - ExperimentalCulture
    range: string
  growth_time:
    name: growth_time
    description: 'Total growth time for the culture.

      Required for ExperimentalCulture activities.'
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: growth_time
    owner: ExperimentalCulture
    domain_of:
    - ExperimentalCulture
    range: string
  organism_ref:
    name: organism_ref
    description: 'FK reference to an organism representing the biological identity

      strain, isolate, engineered construct) that this sample or activity

      is associated with.'
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    aliases:
    - strain_ref
    - strain_id
    rank: 1000
    alias: organism_ref
    owner: ExperimentalCulture
    domain_of:
    - CultureGrowth
    - AMP2UserSample
    - EngineeredStrainSample
    range: organism
    required: false
  growth_medium:
    name: growth_medium
    description: Method of growth and medium/materials used. Indicate broth, gel,
      3-D structure, bioreactor, etc. followed by the formula, recipe, or components
      used to create the growth medium.
    title: growth medium
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: growth_medium
    owner: ExperimentalCulture
    domain_of:
    - CultureGrowth
    - CultureEnvironmentalSample
    - FieldDeployedTerraformSample
    - MixedCultureSample
    - OtherUndescribedSample
    - PureCultureSample
    - TerraformSample
    range: string
  incubation_time_hours:
    name: incubation_time_hours
    description: Incubation duration in hours
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: incubation_time_hours
    owner: ExperimentalCulture
    domain_of:
    - CultureGrowth
    range: float
  container_type:
    name: container_type
    description: Physical container used for the culture or sample (flask, tube, plate,
      etc.)
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: container_type
    owner: ExperimentalCulture
    domain_of:
    - ContainerType
    - CultureGrowth
    - FiltrationProcess
    range: string
  temperature_celsius:
    name: temperature_celsius
    description: Temperature at which the method/process/activity was performed
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: temperature_celsius
    owner: ExperimentalCulture
    domain_of:
    - RIDataGenerationActivity
    - ChromatographyConfiguration
    - HasIncubationConditions
    - ChemicalConversionProcess
    range: float
  agitation_speed_rpm:
    name: agitation_speed_rpm
    description: Agitation/shaking speed in RPM (0 for static)
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: agitation_speed_rpm
    owner: ExperimentalCulture
    domain_of:
    - HasIncubationConditions
    range: integer
  oxygen_saturation_pct:
    name: oxygen_saturation_pct
    description: Percent saturation of oxygen in the incubation atmosphere (0–100).
      For example, ambient air is approximately 20.9%.
    title: oxygen saturation percent
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: oxygen_saturation_pct
    owner: ExperimentalCulture
    domain_of:
    - HasIncubationConditions
    range: float
  name:
    name: name
    description: Human-readable name for the entity or activity.
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: name
    owner: ExperimentalCulture
    domain_of:
    - Activity
    - Entity
    - DataProduct
    - DataGenerationActivity
    - Instrument
    - OntologyClass
    - ContainerAxis
    - SampleProcessing
    - Configuration
    - CalibrationStandardRun
    - PurchasedMaterial
    - organism
    - Site
    - Sample
    - SamplingActivity
    - SoilSamplingActivity
    - SampleProcessingProtocol
    - SampleProcessingRun
    - Study
    - SoftwareControlledTermValue
    range: string
    required: true
  description:
    name: description
    description: Human-readable description for the entity or activity
    title: description
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: description
    owner: ExperimentalCulture
    domain_of:
    - Activity
    - Entity
    - DataProduct
    - DataGenerationActivity
    - DataProcessingActivity
    - OntologyClass
    - ContainerType
    - LabDevice
    - SampleProcessing
    - Configuration
    - CalibrationStandardRun
    - PurchasedMaterial
    - organism
    - Site
    - Sample
    - SamplingActivity
    - SoilSamplingActivity
    - SampleProcessingProtocol
    - SampleProcessingRun
    - Study
    - TimestampValue
    - TextValue
    - SoftwareControlledTermValue
    - ControlledTermValue
    - QuantityValue
    range: string
  in_protocol:
    name: in_protocol
    description: 'The SampleProcessingProtocol (the recipe) that this step follows.
      Type-level, not instance-level: every execution of the same SOP points at the
      same record, so this does NOT identify a particular chain. Use in_run for that.
      A chain may mix protocols, so this is recorded per step rather than per run.'
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    rank: 1000
    alias: in_protocol
    owner: ExperimentalCulture
    domain_of:
    - SampleProcessing
    range: SampleProcessingProtocol
  id:
    name: id
    from_schema: https://emsl-computing.github.io/BASALT-Schema
    identifier: true
    alias: id
    owner: ExperimentalCulture
    domain_of:
    - Activity
    - Entity
    - DataProduct
    - DataGenerationActivity
    - DataProcessingActivity
    - AlternativeIdentifier
    - FunctionalAnnotationIdentifier
    - Instrument
    - OntologyClass
    - ContainerType
    - Custodian
    - InstrumentAlternativeIdentifier
    - LabDevice
    - SampleProcessing
    - ProcessingSampleLink
    - Configuration
    - MobilePhaseSegment
    - MobilePhase
    - MobilePhaseSubstance
    - CalibrationStandardRun
    - PurchasedMaterial
    - MAOMProduct
    - WEOMProduct
    - organism
    - Site
    - Sample
    - AerosolArmSample
    - AerosolSample
    - AMP2UserSample
    - CommerciallyPurchasedSample
    - CultureEnvironmentalSample
    - EngineeredStrainSample
    - FieldDeployedTerraformSample
    - MixedCultureSample
    - MonetSoilSample
    - OtherUndescribedSample
    - PlantSample
    - PureCultureSample
    - SedimentSample
    - SoilSample
    - SynthesizedMaterialSample
    - TerraformSample
    - WaterSample
    - ProcessedSample
    - CoreSection
    - SamplingActivity
    - AerosolArmSamplingActivity
    - AerosolSamplingActivity
    - CommerciallyPurchasedSamplingActivity
    - CultureEnvironmentalSamplingActivity
    - EngineeredStrainSamplingActivity
    - FieldDeployedTerraformSamplingActivity
    - MixedCultureSamplingActivity
    - MonetSoilSamplingActivity
    - OtherUndescribedSamplingActivity
    - PlantSamplingActivity
    - PureCultureSamplingActivity
    - SedimentSamplingActivity
    - SoilSamplingActivity
    - SynthesizedMaterialSamplingActivity
    - TerraformSamplingActivity
    - WaterSamplingActivity
    - SampleProcessingProtocol
    - SampleProcessingRun
    - SubstancesUsedLink
    - SampleProcessingSubstance
    - Study
    - ProjectParticipant
    - TimestampValue
    - TextValue
    - SoftwareControlledTermValue
    - ControlledTermValue
    - PersonValue
    - QuantityValue
    - ConditioningValue
    - zipDownload
    range: uuid
    required: true